Submitted: 17 Dec 2020
Revision: 09 Feb 2021
Accepted: 14 Mar 2021
ePublished: 22 Mar 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Int J Phytocos Nat Ingred. 2021;8(1): 3.
doi: 10.15171/ijpni.2021.03
  Abstract View: 1427
  PDF Download: 696

Original Research

Investigation of acetylcholinesterase, butyrylcholinesterase, α-glucosidase, α-amylase, and tyrosinase inhibition and antioxidant activity of methanol and water extracts from aerial parts of Phlomis lycia D. DON

Nuraniye Eruygur* ORCID logo, Fatma Ayaz ORCID logo

1 Department of Pharmacognosy, Selcuk University, 42250, Konya, Turkey


Background: Natural antioxidants in the plant kingdom play an essential role in reducing the risk of various chronic diseases and maintaining a healthy life. In addition, acetylcholinesterase, butyrylcholinesterase, α-glucosidase, α-amylase, and tyrosinase are involved in the occurrence and pathology of some as Alzheimer’s and diabetes, have become an important strategic target in the effective treatment of these diseases. Phlomis lycia D. DON is traditionally used as an appetizer, stimulant, carminative, tonic, and against stomach pain and dyspeptic complaints. There is limited knowledge on this species except for preliminary studies such as botanical and phytochemicals and biological properties. The aerial parts of this plant contained phenolic compounds, such as lignan, phenylethanoid, and iridoid derivatives which can be responsible for the potential biological effects. Therefore, the present study was aimed to investigate the antioxidant and enzyme inhibition activity of the methanol and water extracts obtained from aerial parts of P. lycia in the flora of Turkey.
Material and Methods: Antioxidant potentials were determined spectrophotometrically by DPPH and ABTS radical scavenging, iron chelating, and β-Carotene/linoleic acid emulsion method. We evaluated the inhibitory activities of methanol and water extracts of P. lycia against various enzymes (acetylcholinesterase, butyrylcholinesterase, α-glucosidase, α-amylase, and tyrosinase) by using 96-well plate methods.
Results: According to the results, the methanol extract was more active than water extract in terms of antioxidant activity. On the other hand, although the methanol and water extracts were observed to demonstrate similar enzyme inhibitory activity, the water extract exhibited more inhibition activity on α-glucosidase.
Conclusions: Our results suggested that Phlomis lycia could potentially be used for the isolation of potent antioxidants from the methanol extract. To the best of our knowledge, this study represents the first time that Phlomis lycia was reported in the literature with glucosidase inhibition activity.
Keywords: Phlomis lycia, Antioxidant activity, Enzyme inhibition, α-glucosidase, α-amylase
First Name
Last Name
Email Address
Security code

Abstract View: 1427

Your browser does not support the canvas element.

PDF Download: 696

Your browser does not support the canvas element.