Submitted: 14 Feb 2018
Revision: 25 Jun 2018
Accepted: 16 Jul 2018
ePublished: 23 Jul 2018
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Int J Phytocos Nat Ingred. 2018;5(1): 2.
doi: 10.15171/ijpni.2018.02
  Abstract View: 4508
  PDF Download: 3156

Original Research

Anti-urease activity of native species of genus Piper from Guatemala with potential application in infection control

Armando Cáceres 1,2*, Fabiana Almeda 1, Laura M. Astorga 1, Andrea C. Orellana 1, Ligia I. Sampuel 1, Paola Sierra 1, Vivian F. Zelada 2

1 Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala
2 Facultad de Biología y Farmacia, Universidad Galileo, Guatemala


Introduction: Urease is an enzyme that catalyzes the hydrolysis of urea in the gastrointestinal and urinary systems, related with chronic bacterial infection. Piper is a basal angiosperm genus with ethnobotanical application. The aim of this study was to determine urease inhibitory activity of extracts from ten Guatemalan Piper species.

Methods: Leaves from 7 species were collected in Suchitepequez (P. amalago, P. auritum, P. hispidum, P. jacquemontianum, P. oradendron, P. retalhuleuense, and P. umbellatum), and 3 in Alta Verapaz (P. psilorhachis, P. sempervirens, and P. variabile), shade-dried and milled. Dichloromethane (DCM) and methanol (MeOH) extracts were prepared by percolation and concentrated by rotavapor. The inhibitory effect was determined by enzymatic assays, qualitative by thin layer chromatography developed by phenol red; and quantitative by spectrophotometric kinetic method evaluated by microcolorimetry at 630 nm with phenol red.

Results: Seventeen extracts out of 20 demonstrated anti-urease activity by TLC, showing one discoloration band with Rf 1.4-5.8; P. psilorhachis showed 2 discolorations bands at Rf 5.3 and 5.8, and the best activity (IC50 MeOH 1.9 ± 0.08 μg/mL and DCM 2.1 ± 06 μg/mL), similar to positive control (hydrocyclohexanone IC50 1.4 ± 0.05 μg/mL). Two other species, P. umbellatum (IC50 MeOH 2.5 ± 0.06 μg/mL) and P. retalhuleunse (IC50 MeOH 4.1 ± 0.7 μg/mL, DCM 4.8 ± 0.06 μg/mL) showed a moderate activity.

Conclusion: Three species showed interesting anti-urease activity that deserves future studies by bio-guided fractionation. Literature review demonstrated that this is the first report about the urease activity of these Piper species.

Keywords: Urea, Piper psilorhachis, Piper umbellatum, Piper retalhuleuense, Helicobacter pylori
Please cite this paper as: Cáceres A, Almeda F, Astorga LM, et al. Anti-urease activity of native species of genus Piper from Guatemala with potential application in infection control. Int J Phytocos Nat Ingred. 2018;5:2. doi:10.15171/ijpni.2018.02.
First Name
Last Name
Email Address
Security code

Abstract View: 4508

Your browser does not support the canvas element.

PDF Download: 3156

Your browser does not support the canvas element.