Marcio Campos
1, Maíra Uliana
2, Daniel V. Montero
1*, Giuseppina Lima
2, Lin Chau Ming
11 Laboratory of Medicinal Plants and Ethnobotany, Department of Horticulture, Faculty of Agronomic Science, State University of São Paulo (UNESP), Botucatú, Brazil
2 Laboratory of Chemistry and Biochemistry, Institute of Bioscience, State University of São Paulo (UNESP), Botucatú, Brazil
Abstract
The objective of this study was to
evaluate the growth of biomass of Passiflora
incarnata L. in response to different levels of organic fertilizer and
compare the content of total polyphenols, total flavonoids and TEAC in leaves
and stems. The experiment was conducted with family farmers in Botucatu
(Brazil), between October 2013 and February 2014. Seedlings were grown in a
greenhouse and then transplanted to the field with spacing 0.30m x 0.60m
without staking and irrigated by sprinkler. The experimental design was a
randomized block design with five treatments and four blocks. The organic
fertilization and liming were made in the row with rabbit manure, chicken
manure, organic waste and dolomitic limestone 30 days before transplanting. Leaves
and stems of P. incarnata were dried
in oven with air circulation at 40 °C / 48 h. The analysis of total flavonoids and
polyphenols were performed according to the spectrophotometric method using the
Folin-Ciocalteau reactive. Results showed that organic fertilization had a positive
effect on the plant´s development, incising the biomass production (dry matter).
However, the production of bioactive compounds such as polyphenols, total
flavonoids and the antioxidant capacity were not influenced by the effect of
organic fertilization.
Keywords: medicinal plant, passion fruit, phytochemistry, flavonoids, polyphenols, antioxidant