Yann-Olivier Hay
1,2,3,4*, Miguel Antonio Abril Sierra
2, Mayra Tellez
1, Luis G. Sequeda C
1, Alba N. Tellez A
1, Catherine Bonnafous
5, Christine Raynaud
3,41 Grupo de Investigación Fitoquímica Universidad Javeriana, Departamento de Química, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Edificio Carlos Ortiz S.J. (52), Laboratorio 210, Bogotá, Colombia
2 Farmaverde CTA, Bogota, Colombia
3 Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), F-31030 Toulouse, France
4 INRA, UMR1010-CAI, F-31030 Toulouse, France
5 Action Bio, Tantène, 46170 Pern, France
Abstract
Two
procedures of steam distillation of Rosmarinus
officinalis and Thymus vulgaris
were compared by phytochemical, antioxidant and antimicrobial analysis of their
respective essential oils and hydrosols using respectively GCFID and GCMS
analysis, free radical scavenging activity on ABTS and dilution and diffusion
method against 3 bacteria and 2 fungi. Differences in bioactivity results are
discussed taking into account changes in molecular content of essential oils
and hydrosols obtained by the two different distillation methods. Although no
significant difference was observed between the two procedures with Rosmarinus officinalis, that was not the
case with Thymus vulgaris.
Significant differences were noted, not only in their respective chemical
composition especially with the thymol and p-cymene percentage with
respectively a change from 39% with conventional steam distillation to 27% with
adapted steam distillation for thymol and from 28% with the first distillation
to 37% with the second one for p-cymene. These phytochemical composition
changes have an impact on their biological activities results and consequently
on the therapeutic activities expected which shows the importance of
distillation method selection.
Keywords: Rosmarinus officinalis, Thymus vulgaris, essential oil, hydrosol, antioxidant, antimicrobial