Submitted: 15 Mar 2014
Accepted: 31 May 2014
First published online: 19 Sep 2014
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Int J Phytocos Nat Ingred. 2014;1(1):5.
doi: 10.15171/ijpni.2014.05
  Abstract View: 2280
  PDF Download: 1607

Original Research

Chemical composition, anti-dermatophytic activity, antioxidant and total phenolic content within the leaves essential oil of Vitex trifolia


This study was carried out to determine the contents of total phenol as well as antioxidant activity, the chemical composition and anti-dermatophytic activity of the Vitex trifolia (Family: Lamiaceae) essential oil of the leaves. Hydro-distillation of the V. trifolia was carried out using Clavenger Vendor to obtain the volatile oil. Total phenolic content and antioxidant activity were determined using the Folin-Ciocalteau reagent and DPPH radical scavenging assay test by spectrophotometric methods. Gas chromatography- mass spectrometric (GC-MS) analyses (BP - 1 chrompack capillary column) of the essential oil was performed and its composition determined. The in-vitro anti-dermatophytic activity was evaluated both by agar diffusion and microbroth dilution methods against Candida albicans ATCC 90029, C. albicans ATCC 1162, C. albicans ATCC Y-9-19, C. parapsilosis ATCC 20019, C. glabrata ATTC 91030, C. krusei ATCC 6258, C. krusei ATCC 71061-1113 and C. kefyr ATCC 1110. The fresh leaves were yielded 0.21% of yellowish colour essential oil. By GC-MS analysis of the essential oil, 50 constituents were identified. 1R-α-pinene, Ocimene, 3-carene, Toluene, p-ethyl-, Eucalyptol and 3-methoxy-5-methylphenol were the major constituents. The V. trifolia contained appreciable levels of amount of total phenolic content (74.66μg GAE/100mg) of the fresh weight and the DPPH free radical scavenging activity (the IC50 = 5.2 μg/ml). The anti-dermatophytic activity showed that essential oil had some antifungal activity on the tested Candida in the concentration 500 μg/ml. The highest antifungal activity was C. krusei ATCC 6258 and C. kefyr ATCC 1110 (zone diameter 27.97±0.4 mm and 23.88±0.8 mm) respectively and C. krusei ATCC 6258 (MIC = 1.953125 μl/ml).
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