Submitted: 13 Mar 2014
Accepted: 24 May 2014
First published online: 10 Sep 2014
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Int J Phytocos Nat Ingred. 2014;1(1):1.
doi: 10.15171/ijpni.2014.01
  Abstract View: 2026
  PDF Download: 1450

Original Research

Antioxidant activity of phenolic compounds from whole cottonseed by-product

Diana Yamile Gallego Villa, Anna Lisa Piccinelli, Luigi Russo, Luca Rastrelli *

1 Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Cra 30 Nº 45-03, Bogotá, Colombia
2 Dipartimento di Farmacia, University of Salerno, Via Ponte don Melillo, 84084, Fisciano (SA), Italy


Cottonseeds are fed to high-producing dairy cows as a source of fat and highly-digestible fiber. Previously we reported the isolation and structure characterization of different phenolic compounds, quercetin, kaemferol and benzoic acid derivatives (1-9) from whole cottonseed by-product. In this paper the antioxidant activity of all isolated compounds was determined by measuring free radical scavenging effects using the Trolox equivalent antioxidant capacity assay and the coupled oxidation of β-carotene and linoleic acid (autoxidation assay). The range of phenolic compounds present in WCS (1.90 g/kg), assayed by the Folin-Ciocalteu method, and their antioxidant properties provides a valuable database for possible nutritional studies on the impact of diet on animal health and productivity.
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